RAPID DETECTION OF COVID-19 VIRUS BY DIGITAL PCR BASED ON MICROCAVITY ARRAY

Nucleic acid amplification detection is one of the most widely used molecular diagnostic techniques in recent years, which can rapidly and efficiently amplify the characteristic nucleotide sequences of pathogenic bacteria in the diagnosis of infectious diseases, it has been widely used in clinical diagnosis, disease screening and other fields. In this work, we report a micro-cavity digital PCR for rapid detection of pathogens on a silicon-based microfluidic chip. The device has the advantages of high flux, no pumping, rapid reaction, quantification and high sensitivity. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.